Figure 2.
Schematic representation of the subcloning of ejh‐ejl cassette in the chromosome of P. putida KT2440 under transcriptional control of the positively regulated Pm promoter. Plasmids are drawn with the relevant elements and restriction sites indicated, as well as the direction of transcription of the genes. White arrows represent the Pm promoter of the meta‐pathway operon of the TOL plasmid. The ejh and ejl genes are shown as black and white blocks boxes respectively. xylS gene, encoding the cognate regulator of Pm, is indicated as a dotted block. Dark grey blocks indicate the antibiotic resistance (KmR indicates the gene conferring kanamycine resistance). The regions of the plasmids encoding the Tn5 transposase gene and the beta‐lactamase gene are also indicated.