Table 1.
Some physiological and phylogenetic characteristics of the novel bacteria isolated from bioreactor I and II.
| Isolate | Bioreactor pHa | Major physiological characteristics | Amplification product length (nt) (16S rRNA gene) | GenBank accession No. | T‐RF lengthb (nt) | Nearest relative | Identity (%) |
|---|---|---|---|---|---|---|---|
| CEB3 | 2.4 | Spore‐forming motile rods; obligately anaerobic sulfidogen | 1314 | JF346160 | 138 | Uncultured Desulfitobacterium sp. clone E41 (Winch et al., 2009) | 95.0 |
| IR1 | 4.0 | Rod‐shaped motile cells; spores not observed; facultative anaerobe; non‐sulfidogen | 1303 | JF346161 | 231 | Uncultured Actinobacterium clone (Akob et al., 2008) | 98.0 |
| IR2 | 2.2 | Spore‐forming motile rods; facultative anaerobe; non‐sulfidogen | 1302 | JF346159 | 212 | Uncultured Alicyclobacillus sp. clone (G13_bac) (Winch et al., 2009) | 99.0 |
a.
At the time of isolation.
b.
Digested with the restriction enzyme HaeIII.