Table 1.
Relative amount of 32:1/34:1/34:2 (%) | Relative amount of 16:0/16:1/18:0/18:1 (%) | |||
---|---|---|---|---|
PE | PG | Total glycerophospholipids | ||
P. putida KT2440 | ||||
0% n‐butanol non‐treated | 81 | 85 | 78 | 93 ± 6 |
0% n‐butanol treated | 81 | 84 | 78 | 82 ± 6 |
1% n‐butanol non‐treated | 81 | 75 | 75 | 99 ± 0 |
1% n‐butanol treated | 84 | 14 | 52 | 99 ± 0 |
P. putida DOT‐T1E | ||||
0% n‐butanol non‐treated | 78 | 84 | 78 | 99 ± 0 |
0% n‐butanol treated | 79 | 84 | 78 | 99 ± 0 |
1% n‐butanol non‐treated | 12 | 6 | 9 | 54 ± 1 |
1% n‐butanol treated | 83 | 88 | 81 | 98 ± 2 |
P. putida S12 | ||||
0% n‐butanol non‐treated | 81 | 78 | 77 | 99 ± 0 |
0% n‐butanol treated | 81 | 78 | 78 | 99 ± 0 |
1% n‐butanol non‐treated | 77 | 57 | 61 | 95 ± n.d. |
1% n‐butanol treated | 82 | 66 | 73 | 99 ± 0 |
Pseudomonas sp. VLB120 | ||||
0% n‐butanol non‐treated | 79 | 82 | 76 | 99 ± 0 |
0% n‐butanol treated | 78 | 82 | 76 | 99 ± 0 |
1% n‐butanol non‐treated | 80 | 75 | 72 | 98 ± 0 |
1% n‐butanol treated | 79 | 81 | 76 | 99 ± 0 |
The relative amounts of 32:1/34:1/34:2 phosphatidylethanolamine and phosphatidylglycerol were calculated from their abundance within the respective glycerophospholipid class multiplied with the relative amount of these classes. The relative amount of the major fatty acid moieties 16:0/16:1/18:0/18:1 was calculated for the total glycerophospholipid pool. Free fatty acids were not considered. Errors represent the standard deviation of independent experiments (n = 2–8). n.d.: not determined because of missing replicates.