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Primers used in this study.
| Primers | Sequence (5′→3′) |
|---|---|
| alk_P_up | GTCGTACCATCCTGTAATGTGAAATGAG |
| alk_EB_reva | CATCCAGAATTCGGATCCTAAGTAACGTTT |
| alk_EB_fora | AAACGTTACTTAGGATCCGAATTCTGGATG |
| alk_out_down | CGAGCGCCAAATGAGACAGGATATGA |
| ADP1_alk_for | TTGATTAAGCGGATGGCTGGCATATA |
| luxC_rev | GAGAGTCATTCAATATTGGCAGG |
| ADP1_alk_rev | CGAACTATGAAAAGCAGCACTCATCC |
The underlines indicate EcoRI and BamHI restriction sites.
