Figure 3.

Influence of overexpression of genes for anaplerotic enzymes in C. glutamicum strain BL‐1 on biomass formation (A), glucose consumption (B), succinate production (C) and acetate production (D). The following strains were tested: BL‐1 (squares), BL‐1 with pAN6‐pyc^P458S (pyruvate carboxylase, circles), BL‐1 with pAN6‐pyc^P458Sppc (pyruvate carboxylase and PEP carboxylase, triangles pointing upwards) and BL‐1 with pAN6‐aceAaceB (isocitrate lyase and malate synthase, triangles pointing downwards). The strains were cultivated in modified CGXII medium containing 4% (w/v) glucose, 25 mg l⁻¹ kanamycin and 0.5 mM IPTG under constantly control conditions of pH 7.0 and pO₂ > 30% using a Multifors bioreactor system. Mean values from two independent cultivations are shown.