Figure 5.

Growth (squares), glucose consumption (circles), succinate production (diamonds) and acetate production (triangles) of C. glutamicum BL‐1 with pAN6‐pyc^P458Sppc under conditions of nitrogen excess (open symbols) and nitrogen limitation (solid symbols) during batch cultivation in modified CGXII medium containing 4% (w/v) glucose, 25 mg l⁻¹ kanamycin and 0.5 mM IPTG under constantly control conditions of pH 7.0 and pO₂ > 30% in a Multifors bioreactor system. Under nitrogen excess, the medium contained 151 mM (NH₄)₂SO₄ and no urea; under nitrogen limitation, the medium contained no urea and 20 mM (NH₄)₂SO₄. Mean values from at least two independent cultivations are shown.