Table 1.
Growth and organic acid production parameters of different C. glutamicum succinate producer strains during aerobic cultivation in modified CGXII medium containing 4% (w/v) glucose, 25 mg l−1 kanamycin (plasmid‐containing strains only) and 0.5 mM IPTG (plasmid‐containing strains only) at pH 7.0 and pO2 > 30% saturation.
| C. glutamicum strain | Growth rate (h−1) | Biomass [g (cdw) l−1] | Glucose consumed (mM) | Glucose uptake rate [mmol g (cdw)−1 h−1] | Final acetate titre (mM) | Final succinate titre (mM) | Succinate production rate [mmol g−1 (cdw) h−1] | Yield succinate/glucose (mol mol−1) |
|---|---|---|---|---|---|---|---|---|
| Wild type | 0.34 | 18.1 | 210 | 4.30 | n.d. | n.d. | n.d. | n.d. |
| Δsdh | 0.31 | 13.1 | 225 | 5.29 | 125 | 40 | 0.75 | 0.18 |
| BL‐1 | 0.33 | 13.0 | 233 | 5.54 | 22 | 66 | 1.39 | 0.28 |
| BL‐1 (pAN6‐pycP458S) | 0.30 | 12.2 | 242 | 5.49 | 20 | 68 | 1.44 | 0.28 |
| BL‐1 (pAN6‐pycP458Sppc) | 0.29 | 12.2 | 230 | 5.15 | 20 | 82 | 1.60 | 0.36 |
| BL‐1 (pAN6‐aceAaceB) | 0.27 | 10.5 | 235 | 5.19 | 22 | 72 | 1.38 | 0.30 |
All parameters describing rates were calculated for the exponential growth phase of the cultures. The data presented are mean values from at least two independent batch cultivations.