Table 2.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristic(s) | References and comments |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F‐(ϕ80dΔlacZ M15)Δ(lacZYA‐argF) U169hsdR17(r‐M) recA1 endA1 relA1 deoR)λ‐, phoA supE44thi‐1, gyrA96 | Hanahan (1983) |
| S. aureus | ||
| RAB171 | SA113 fdh::Pxyl/tet‐gfpmut2 | Stary et al. (2010) |
| RAB200B | SA113 lip::Pt17‐tetR(B), lox66‐aphAIII‐lox71 | This study |
| RAB201B | SA113 lip::Pt17‐tetR(B), lox72 | This study |
| RAB210B | SA113 lip::Pt17‐tetR(B), lox66‐aphAIII‐lox71, fdh::Pxyl/tet‐gfpmut2 | This study |
| RAB211B | SA113 lip::Pt17‐tetR(B), lox72, fdh::Pxyl/tet‐gfpmut2 | This study |
| RN4220 | NCTC8325‐4 derivative, acceptor of foreign DNA | Iordanescu and Surdeanu (1976) |
| SA113 (ATCC 35556) | NCTC8325 derivative, agr‐, 11 bp deletion in rbsU | Iordanescu and Surdeanu (1976) |
| Plasmids | ||
| pJL71 | pCN54 derivative (Charpentier et al., 2004) bearing gp‐mcherry | This study |
| pKX15 | aphAIII, xylR, PxylA, pTX15 derivative (Peschel et al., 1996) | This study, aphAIII cloned from pDG782 (Guérout‐Fleury et al., 1995) via StuI and Acc65I |
| pRAB2‐B | cat, bla, ‘lip’‐Pt17‐tetR(B)‐lox66‐aphAIII‐lox71‐‘lip’ pBT2 derivative (Brückner, 1997) | This study, tetR(B) cloned from pWH1925‐derivative (Scholz et al., 2004) via XbaI and BstEII |
| pRAB32‐gp/‐ntgp/‐ctgp | pKX15 derivative bearing native, N‐terminally or C‐terminally tip‐tagged gp‐mcherry | This study, gp‐mcherry cloned from pJL71, attachment of tip‐tags by commercially purchased DNA fragments (Entelechon, Regensburg) |
Antibiotics were used, where appropriate, at the following final concentrations: ampicillin 100 µg ml−1, chloramphenicol 10 µg ml−1 and kanamycin 15 µg ml−1.