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Amino acid substitutions of thermostable GA mutants.
| Mutant | Amino acid substitutions | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N20C | A27C | S30P | V59A | T62A | V88I | S119P | G137A | S211P | T290A | D293A | T390S | H391Y | Y402F | E408K | S436P | |
| RE5 | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | |||||||||
| RE15 | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ||||||||
| SRE15 | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ||||
| CR1‐1 | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ||||||
| CR2‐1 | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ | ✗ |
