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Parameters for irreversible thermoinactivation of wild‐type and mutant GAs.
| GA form | ΔG72.5°C (kJ mol−1) | ΔG‡80°C (kJ mol−1) | Tm (°C) |
|---|---|---|---|
| Wild type | 101 | 96 | 69.2a |
| RE5 | 107 | 101 | 74.6 |
| RE5+E408K | 108 | 103 | 75.7 |
| CR1‐1 | 107 | 103 | 75.4 |
| RE15 | 105 | 101 | 72.8 |
| SRE15 | 106 | 104 | 75.0 |
| SRE15+V59A | 110 | 105 | 78.5 |
| CR2‐1 | 111 | 106 | 79.2 |
Temperature at which enzymes are 50% inactivated after 10 min.
