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. 2013 Nov 1;8(11):e77899. doi: 10.1371/journal.pone.0077899

Figure 4. Effects of BCAA supplementation on angiogenesis in rats after 16 weeks of DEN administration.

Figure 4

The relative band intensities of the angiogenesis markers are shown in the right-hand panels (A). The expression level of Tie-2 mRNA was determined by RT-PCR (B). VEGF protein expression was determined by Western blotting (C). The expression level of each gene is normalized to those of GAPDH (A and B) and β-actin (C). The lanes contain mRNA (A) and protein (C) samples from three rats per group. Values shown are means ± standard deviation. *P<0.05.