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. 2013 Nov 1;8(11):e78417. doi: 10.1371/journal.pone.0078417

Figure 5. Revivification of RhoB gene and apoptosis of ovary carcinoma cells.

Figure 5

Flow cytometric analysis (FCM) together with fluorescence microscopy was adopted to assess apoptosis of ovary carcinoma cells after treated with TSA. Top and middle panels: when cells were treated for 10 h with 0 µmol/L (control), 0.05 µmol/L, 0.1 µmol/L, 0.25 µmol/L, 0.5 µmol/L and 1.0 µmol/L of TSA, the apoptosis rate revealed by FCM was 9.5% (A), 26.9% (B), 28% (C), 41% (D), 45.9% (E) and 66.9% (F) respectively. Bottom panel: PI stained fluorescence photomicrographs of ovary carcinoma cells after treated with TSA. Cells were treated for 10h with 0 µmol/L (G, control), 0.1 µmol/L and 0.5 µmol/L of TSA respectively, both 0.1 µmol/L (H) and 0.5µmol/L (I) of TSA could result in cellular morphological changes characterized as apoptosis: a brightly red-fluorescent condensed nuclei (intact or fragmented), reduction of cell volume, and apoptotic bodies.