Table 3.

Copy numbers and accumulation levels of 16S rRNA of microorganisms present in the industrial heap (samples of August 2007) by real‐time PCR analysis.

Microorganism	Copy numbers (copies ml−1)			Transcripts levels (copies ml−1)
	Strips			Strips
	S10	S8	S5	S10	S8	S5
Bacteria	6.7 × 106	9.0 × 105	3.2 × 105	ND	ND	ND
At. ferrooxidans D2	3.4 × 106	3.1 × 105	1.2 × 103	1.1 × 107	5.9 × 104	2.1 × 104
At. ferrooxidans DM	2.9 × 106	8.1 × 104	2.3 × 103	1.0 × 107	4.1 × 104	4.7 × 104
At. thiooxidans	1.3 × 105	8.9 × 104	3.4 × 104	6.9 × 105	7.5 × 104	2.7 × 105
L. ferriphilum	3.8 × 103	1.9 × 104	1.4 × 105	5.3 × 102	3.8 × 103	2.2 × 105
L. ferriphilum Warwick	2.2 × 103	4.2 × 105	5.6 × 104	1.5 × 103	4.0 × 104	2.8 × 104
Ab. disulfidooxidans	7.4 × 103	2.1 × 104	2.7 × 103	3.3 × 103	1.3 × 102	3.3 × 103
Sulfobacillus‐like	8.9 × 102	6.0 × 102	BDL	BDL	1.9 × 102	1.3 × 103
Uncultured Alicyclobacillus	2.2 × 102	1.1 × 102	BDL	BDL	BDL	BDL
Acidiphilum‐like	9.6 × 103	9.2 × 103	BDL	5.1 × 104	2.9 × 104	1.4 × 103
Archaea	BDL	2.0 × 102	2.0 × 103	ND	ND	ND
F. acidiphilum	BDL	1.4 × 102	2.1 × 103	4.1 × 102	2.2 × 103	1.3 × 104

BDL, below detection limit. ND, not done.