Figure 2.

Schematic representation of relevant regions of Bbr_0838 gene in B. breve UCC2003 and B. breve UCC2003::838 chromosomes. A. Chromosomal DNA is represented by a thin line, Bbr_0838 by a black arrow, Bbr_0838 fragment employed for homologous recombination by a grey bar and pORI19 by a dashed line. Representative EcoRI sites for Southern hybridization are indicated. B. Southern hybridization of EcoRI digested chromosomal DNA from B. breve UCC2003 (lane 1) and 8 representative insertional mutants (lanes 2 to 4 correspond to UCC2003::838₈₀₀ clones and lanes 5 to 9 correspond to UCC2003::838₁₀₀₀clones) are shown in B. Sizes of hybridized fragments are shown at the left of the panel. pORI19‐838₁₀₀₀‐tetW was used as a probe and positive control of hybridization was performed on lane 10.