Table 2.
Primers used in this study
| Sequence | Source | |
|---|---|---|
| Promoter fragments | ||
| Prom838F2bglII | 5′‐GCTCGTAGATCTGCCTCGCATGTTCAGCTTCAC‐3′ | This work |
| Prom838FbglII | 5′‐GCTCGTAGATCTCATAGCCATCAACTTACG‐3′ | This work |
| Prom838F2BglIIshort | 5′‐CGAGTCAGATCTGGCATGCGTGGGTTTCAC‐3′ | This work |
| Prom838revBamHI + IR | 5′‐CGCATAGGATCCGATGAGTCCGGACGTAAAGG‐3′ | This work |
| Prom838revBamHI‐IR | 5′‐CTGACTGGATCCGCGTAAGTTGATGGCTATG‐3′ | This work |
| Prom838rv + IRII50pstI | 5′‐CGTACAGCTGCAGGATGGGCCATCGGTACATC‐3′ | This work |
| Prom838rv + IRIIpstI | 5′‐CTATAGCCTGCAGCCGCTACACCAGCATCGAG‐3′ | This work |
| Prom838rv‐IRIIpstI | 5′‐GTATACTCTGCAGCACGGCAGGCGCGTGCGATG‐3′ | This work |
| Prom838rv‐pstI | 5′‐CTCACGCTGCAGGTGCGGATCAATAGGCTTTAC‐3′ | This work |
| Insertional mutation | ||
| 838F | 5′‐TGCTCTAAGCTTCTTTCATGGTCGGCACACTG‐3′ | This work |
| 838800R | 5′‐CGCGTCTAGAGAGATGGTCATGAGCGCAAGG‐3′ | This work |
| 8381000R | 5′‐TCAACTTCTAGACATGTGCGAGGCGGTCACG‐3′ | This work |
| Mutant complementation | ||
| pBC‐838‐f | 5′‐CGATCGAAGCCTCGCATGTTCAGCTTCAC‐3′ | This work |
| pBC‐838‐r | 5′‐CGATGCGGATCCTTATCACCGATCATCCTTTGGGAAC‐3′ | This work |
| Primer extension | ||
| 838pseqfor | 5′‐CATCTTGAACGCTTGAATGCCG‐3′ | This work |
| 838pseqrev | 5′‐GCGCCATCCGAAACTGTCAATC‐3′ | This work |
| PE3 | 5′‐GCGTAAGTTGATGGCTATGGTGAG‐3′ | This work |
| PE4 | 5′‐CGATTCATGCGACGTCGACTGC‐3′ | This work |
| Northern probe f | ||
| N838f | 5′‐CCTAGCAAGCTTCGCCGTAGTGCCGATGCT‐3′ | This work |
| N838r | 5′‐GCTCAGTCTAGAGAACGCGATGCCTCTGG‐3′ | This work |
| q‐RT‐PCR | ||
| 406‐fq | 5′‐CACGCTGCTGCATGTAATCG‐3′ | Ruiz and colleagues (2011) |
| 406‐rq | 5′‐GCCAGCCTCGGTCATTTGTA‐3′ | Ruiz and colleagues (2011) |
| 1805‐fq | 5′‐CCTCCAACCGTAACTTCCTGAA‐3′ | Ruiz and colleagues (2011) |
| 1805‐rq | 5′‐CGACCTTGGCCAGCAGTTC‐3′ | Ruiz and colleagues (2011) |
| 1827‐fq | 5′‐CGCGGCTGGCACCAT‐3′ | Ruiz and colleagues (2011) |
| 1827‐rq | 5′‐TCATTGGTCATGTCCTTGAGCTT‐3′ | Ruiz and colleagues (2011) |
| 832‐fq | 5′‐TGCTGCATCTGGTGTTGATCTG‐3′ | Ruiz and colleagues (2011) |
| 832‐rq | 5′‐AAGCCGACCTCCGCATACC‐3′ | Ruiz and colleagues (2011) |
| 1756‐fq | 5′‐CTTCAATGCCACCGTGTATGG‐3′ | Ruiz and colleagues (2011) |
| 1756‐rq | 5′‐CGCCTGCTACTCCGGAAAC‐3′ | Ruiz and colleagues (2011) |
| Bbr838F | 5′‐CGCCCTGATCATCTGCATCT‐3′ | Ruiz and colleagues (2011) |
| Bbr838R | 5′‐TTGCGTGCGGCCTTCTC‐3′ | Ruiz and colleagues (2011) |
Restriction sites are underlined.