Figure 4.

Differential expression of the central catabolic‐pathway (the MMC pathway) enzymes, hydrogenases, and formate dehydrogenases in P. thermopropionicum. Enzymes encoded by the genes in the mmc cluster are indicated with blue letters. Bar graphs represent the log₂ expression ratios of enzymes in a syntrophic co‐culture by reference to the monoculture. For enzymes with multiple genes, mean values are shown. Refer to bottom right of this figure for the x‐ and y‐axes and colours of the bar graphs. Multiple candidate genes are present for Pct (propionate CoA transferase), Adh (alcohol dehydrogenase), Aor (aldehyde oxidereductase) Ald (aldehyde dehydrogenase) and Acs (acetyl‐CoA synthase), and expression profiles for these genes are listed separately (Table S3). Pot, propionyl‐CoA : oxaloacetate transcarboxylase (PTH_1364‐1366, 1368); Mce, methylmalonyl‐CoA epimerase (PTH_1363); Mcm, methylmalonyl‐CoA mutase (PTH_1361‐1362); Scs, succinyl‐CoA synthase (PTH_1358‐1360); Sdh, succinate dehydrogenase (I, PTH_1016‐1018; II, PTH_1492‐1490); Fht, fumarase (PTH_1356‐1357); Mdh, malate dehydrogenase (PTH_1367); Odc, oxaloacetate decarboxylase (PTH_1189‐1190); Ldh, lactate dehydrogenase (PTH_2230), Pfl, pyruvate formate lyase (PTH_2436); Por, pyruvate : ferredoxin oxidoreductase (PTH_1369); Fe‐H₂ase, Fe‐hydrogenase (I, PTH_0668‐0670; II, PTH_1377‐1379; III, PTH_2010‐2012); NiFe‐H₂ase, NiFe‐hydrogenase (PTH_1701‐1704); Fdh, formate dehydrogenase (I, PTH_1711‐1714; II, PTH_2645‐2649).