Table 2.
Kinetic constants of T. versicolor laccase for oxidation of selected natural phenols.
| Plant‐derived phenols | λ (nm) | ε (M−1 cm−1) | Km (µM) | Vmax (U mg−1) | [Substrate]a/Km |
|---|---|---|---|---|---|
| Gallic acid | 306 | 3 200 | 521 ± 75.3 | 1112.8 ± 137.2 | 56.5 |
| Syringic acid | 300 | 8 500 | 11.8 ± 3.3 | 309.1 ± 25.6 | 2144.1 |
| Ferulic acid | 320 | 12 500 | 19.9 ± 4.3 | 719.6 ± 23.9 | 1291.5 |
| Catechin | 450 | 2 200 | 173.0 ± 54.2 | 1843.9 ± 156.1 | 99.6 |
| Catechol | 435 | 2 600 | 362.0 ± 59.5 | 196.4 ± 25.5 | 125.6 |
a.
[Substrate] indicates monomer concentration (µM) used for colouration reactions of hair dyeing.
Means and standard deviations for triplicate samples are shown.