Table 2.
Primers used in this study.
| Primers | 5′‐ to 3′‐sequence, restriction sites underlined | Description |
|---|---|---|
| Hpa1‐F | AACATATGATGAACTCTTTGAACAC | 414 bp hpa1 gene |
| Hpa1‐R1 | TTCTCGAGTTACTGCATCGATCCGCTGTCG | XhoI site was added at 3′‐termi of hpa1 |
| Hpa1‐R2 | TTGGATCCTTACTGCATCGATCCGCTGTCG | BamHI site was added at 3′‐termi of hpa1 |
| P1 | CTCGAGCTACACTTTTTCAATCTTCTTAAAGAGTTTCTGCATCGAT | Sequence for KLFKKIEKV was fused to C‐terminus of Hpa1 |
| P2 | AACTCGAGCTAGAGACCCGTGGTGAGCACCTTAAGCACAGCGACT | Sequence for AVLKVLTTGL was fused to C‐terminus of Hpa1 |
| P3 | CAGGATCCGGGCGGCGGGTTCGGCGGTAAGTGGAAACTCTTTAAG | Sequence for the polylinker DPGGGFGGKW was fused before the α‐helix of cecropin |
| P4 | TTGACCCACTTTTTCAATCTTCTTAAAGAG | Squence for the flexible hinge GQGIG was fused after the α‐helix of cecropin |
| P5 | AACTCGAGCTAGAGACCCGTGGTGAGCACCTTAAGCACAGCGCCA | Sequence for the α‐helix of melittin was fused after the flexible hinge |
| Hin1‐F | GAACGGAGCCTATTATGGCCCTTCC | 867 bp HIN1 gene |
| Hin1‐R | CATGTATATCAATGAACACTAAACGCCGG | |
| HSR203J‐F | TTGAACACACAATTCGGCGG | 618 bp HSR203J gene |
| HSR203J‐R | TTACTGACTCGATGCGCTGTC | |
| PR1‐a‐F | GGCGTTCTCTTTTCACAATTGCCTTCAT | 495 bp PR1‐a gene |
| PR1‐a‐R | AACGGACTTTCGCCTCTATAATTACCTG | |
| EF1a‐F | AGACCACCAAGTACTACTGCAC | 495 bp EF1a gene |
| EF1a‐R | CCACCAATCTTGTACACATCC |