Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Genotype or relevant characteristic(s) | Reference |
|---|---|---|
| Strains | ||
| P. putida | ||
| KT2440R | Wild‐type, Rifr, derivative of KT2440 | Espinosa‐Urgel and Ramos (2004) |
| M717 | trpI::mini‐Tn5‐Km; Kmr, Rifr | Duque et al. (2007) |
| Aux‐1 | Insertion at 7th codon of trpA | This study |
| Aux‐2 | Insertion at 32th codon of trpC | This study |
| Aux‐3 | Insertion at 184th codon of trpD | This study |
| Aux‐4 | Insertion at 57th codon of trpE | This study |
| Aux‐5 | site‐specific homologous inactivation of trpF | This study |
| Aux‐6 | Insertion at 6th codon of pheA | This study |
| Aux‐7 to 9 | Insertion at 37th, 42nd and 120th codon of pheA | This study |
| Aux‐10 | Insertion at 84th codon of tyrA | This study |
| E. coli | ||
| HB101 pRK600 | Host used for cloning assays | Herrero et al. (1990) |
| CC118λpir pUT‐Km | Source of mini‐Tn5; Kmr | Herrero et al. (1990) |
| Plasmids | ||
| pCHESIΩKm | Kmr | Llamas et al. (2003) |
Kmr and Rifr stand for resistance to kanamycin and rifampicin respectively.