Fig. 2.

Cytotoxic effects of F- and Cl-amidine. a MCF7 cells were incubated with various concentrations of F- and Cl-amidine over the course of 24 h, and cell viability was determined by a standard MTT assay. Each data point represents the average of three trials (p < 0.05). Data were normalized to controls treated with PBS only. b Cytotoxic effects of Cl-amidine and ATRA on human HL-60 and HL-60 granulocytes. Cell viability was measured, using a standard MTT assay, after a 24-h incubation with the inhibitors. Each data point represents the average of three trials (p < 0.05). Data were normalized to controls treated with PBS only. c Levels of citrullinated histone H3 in MCF7 cells after treatment with F- and Cl-amidine. See “Materials and methods” section for experimental details. d Levels of citrullinated histone H3 in HL-60 granulocytes after treatment with F- and Cl-amidine. See “Materials and methods” section for experimental details