Skip to main content
. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Anal Chem. 2013 Jul 26;85(15):10.1021/ac401485j. doi: 10.1021/ac401485j

Table 2.

Confirmation of SPION-protein binding situations revealed by the F4-centrifugation method, using CE.

Protein Centri-
fugation?		 F4
Isolation?		 Binding situation
detected by CE		 CE Method Kd(µM)
Antitrypsin X X Slow dissociation CZE 1.01 ± 0.10
Transferrin X X Slow dissociation CZE 0.233 ± 0.011
Haptoglobin X X Slow dissociation CZE 0.612 ± 0.074
Apolipoprotein Al X Fast dissociation ACE 0.155 ± 0.001
Prealbumin Non-binding CZE, ACE Non-binding