FIGURE 3.

Tetramer binding and TNF-α production by CD4 T cells responding to virus infection. A) GP67 tetramer binding by splenocytes was measured at the tetramer dilutions indicated on the x axis, and the values were adjusted for nonspecific tetramer binding. The percent tetramer⁺ cells within the CD44^hiCD4⁺ donor cells at each tetramer concentration was determined at 8 days post-infection (upper panel) and at 65 days postinfection and 5 days following rechallenge (lower panel). B) Splenocytes were cultured for 5h with brefeldin A in the presence of varying concentrations of GP67, and TNF-α production was determined by intracellular staining. Cells were gated on live CD4⁺CD44^hi donor cells. The percent TNF-α⁺ cells within CD4⁺CD44^hi populations was determined at 8 days and 65 days following infection and at 5 days following rechallenge.