Skip to main content
. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: Biochemistry. 2013 Sep 5;52(37):10.1021/bi400965u. doi: 10.1021/bi400965u

FIGURE 5.

FIGURE 5

Spectral and kinetic analyses of heme transfer from ferric holoShp to apoHtsAM79A and apoHtsAH2299A. A, shifts of the absorption spectrum in the reaction of 3.5 μM ferric holoShp with 30 μM apoHtsAM79A in 20 mM Tris-HCl, pH 8.0, at room temperature after mixing in a stopped-flow spectrophotometer. The arrows indicate the directions of the spectral shifts with time. B, absorption spectra of the reaction mixture of 3.5 μM ferric holoShp with 30 μM apoHtsAH229A at the indicated time points after mixing. The spectrum of 3.5 μM holoHtsAH229A (gray line) is included for comparison. C, time course of ΔA402 and ΔA425 at the Soret peak and ΔA630 at the charge transfer bands in the holoShp/apoHtsAM79A reaction as in panel A. The black traces and gray curves represent the observed data and double (for ΔA402 and ΔA425) or single (for ΔA630) exponential fitting curves, respectively.