. Author manuscript; available in PMC: 2014 Sep 17.

Published in final edited form as: Biochemistry. 2013 Sep 5;52(37):10.1021/bi400965u. doi: 10.1021/bi400965u

Table 2.

Number of kinetic phase and rate constants in heme transfer from Shp proteins to HtsA proteins

Donor	acceptor	No. of kinetic phase	k_t (s⁻¹)	k_t1 (s⁻¹)	k_t2 (s⁻¹)	Reference
Shp	HtsA	1	43 ± 3			19
Shp	HtsA^M79A	2		7.4^a	0.8^a	This work
Shp	HtsA^H229A	No transfer				This work
Shp^M66A	HtsA	2		8.7 ± 0.3	0.38 ± 0.08	20
Shp^M66A	HtsA^M79A	1	8.6 ± 0.6^b			This work
Shp^M66A	HtsA^H229A	2		7.0 ± 1.0^c	1.4 ± 0.5^c	This work
Shp^M153A	HtsA	2		120 ± 30	2.5 ± 0.2	20
Shp^M153A	HtsA^M79A	2		8.1 ± 0.4^b	0.48 ± 0.06^b	This work
Shp^M153A	HtsA^H229A	1	14.7 ± 0.3^c			This work

Apparent rate constant obtained at 32 μM apoHtsA^M79A.

Apparent rate constant obtained at 10 μM apoHtsA^M79A.

Apparent rate constant obtained at 13 μM apoHtsA^H229A.