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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Alcohol Clin Exp Res. 2013 Jul 29;37(12):10.1111/acer.12201. doi: 10.1111/acer.12201

FIGURE 4.

FIGURE 4

LCs from mice chronically fed EtOH exhibit reduced MMP2 and MMP9 production. ECS cultures were prepared from mice maintained on EtOH for 32 weeks or controls. Cells were cultured for 24 hours +/− CPG 1826 with addition of GolgiPlug at the end of the culture. (A) MMP2 and MMP9 expression were measured by flow cytometry on gated LC populations. Control for MMP2 (polyclonal unconjugated Ab) is unstained cells with appropriate secondary Ab, and control for MMP9 is isotype control. (B) MFIs from EtOH-fed and control groups for these enzymes. Error bars represent SEM. (***)=P<0.001 for differences between EtOH and control mice within each group. N = 4 mice/treatment group.