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. Author manuscript; available in PMC: 2013 Nov 3.
Published in final edited form as: Cell Metab. 2013 Jan 8;17(1):10.1016/j.cmet.2012.12.004. doi: 10.1016/j.cmet.2012.12.004

Figure 3. Leptin Regulates Bronchial Diameter via a Central Pathway.

Figure 3

(A) Mice with deletion of ObRb in all neurons (ObRbneuron-ERT2−/−) have normal insulin sensitivity (n = 5 per group).

(B and C) ObRbneuron-ERT2−/− mice have decreased R, Rn, and AHR compared to tamoxifen-treated Nestincre-ERT2 and ObRbfl/fl controls (n = 8 per group, *ObRbneuron-ERT2−/− versus Nestincre-ERT2).

(D–F) Leptin (Lep) ICV infusion (4 ng/hr for 5 days) improves baseline R, Rn, and AHR, and endurance in ob/ob, not in db/db mice (n = 8 per group, *ob/ob versus WT, #ob/ob versus ob/ob-Lep).

(G) ObRb localizes in cholinergic-containing nuclei including the dorsal motor vagus (DMVN) (highlighted area) nucleus and facial motor nucleus (Mo7) (arrows) and is absent in mice with an inducible deletion of ObRb in Ach-producing neurons (ObRbAche-ERT2−/−).

(H–J) ObRbAche-ERT2−/− mice have normal insulin sensitivity and increased baseline R, Rn, and AHR compared to tamoxifen-treated Achecre-ERT2 and ObRbfl/fl controls (n = 10 per group). *ObRbAche-ERT2−/− versus Achecre-ERT2.

For all panels, */#p < 0.05, **/##p < 0.01, and ***/###p < 0.001. Error bars represent SEM. See also Figure S3.