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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Stem Cell Res. 2013 Jun 1;11(2):10.1016/j.scr.2013.05.011. doi: 10.1016/j.scr.2013.05.011

Figure 1.

Figure 1

A diagram is shown for the experimental procedure in the derivation of iPS clones. A, schematic experimental procedure. Timed mating was set up between the wild-type DBA/2 female mice (DBA female) and the male mice primarily on a 129 genetic background (129*) carrying the OSKM transgene at the Col1A1 locus on both chromosomes (Carey et al., 2010). E13.5 embryos were used to derive hybrid MEF (DBA/129*) cells carrying one copy of the OSKM transgene from the pregnant female mice. B, an iPS clone (D15D, left panel) with similar morphology to the wild-type TC1 ES cells (ES, right panel). The microscope images are shown for D15D and TC1 on the irradiated SNL feeder cells.