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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Stem Cell Res. 2013 Jun 1;11(2):10.1016/j.scr.2013.05.011. doi: 10.1016/j.scr.2013.05.011

Figure 2.

Figure 2

COBRA analysis of paternally inherited DNA methylation imprint. Genomic DNA was isolated from the iPS clones (Lanes 5–15), mouse tail of the mother (Lane 1) and father (Lane 2), MEF cells without doxycycline (Lane 3), MEF cells with doxycycline (Lane 4) as well as from the wild-type TC1 ES cells (Lane 16). COBRA was performed for three DMR regions (IG-DMR of the Dlk1-Dio3 imprinted region, H19 DMR, Rasgrf1 DMR). The iPS clones used for COBRA are: L7 (Lane 5), L9 (Lane 6), B11 (Lane 7), E7 (Lane 8), D15D (Lane 9), D36 (Lane 10), C8D (Lane 11), E7D (Lane 12), S4D (Lane 13), S5D (Lane 14) and n2 (Lane 15). U, unmethylated product. M, methylated product. A, IG-DMR with TaqI digestion. B, H19 DMR with ClaI digestion. C, Rasgrf1 DMR with BstUI digestion.