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. Author manuscript; available in PMC: 2013 Nov 3.
Published in final edited form as: J Cardiovasc Pharmacol. 2011 Apr;57(4):10.1097/FJC.0b013e31820d92fd. doi: 10.1097/FJC.0b013e31820d92fd

Figure 4.

Figure 4

Effect of EMP1 on apoptosis assessed by surface expression of phosphatidyl serine. HUVECs were subjected to a 24 hour pretreatment with either medium (samples 1 and 2), EPO (5 U/ml) (sample 3), heat-inactivated EPO (sample 4), EMP1 (sample 5) or iEMP1 (sample 6), respectively. Following 4 hours of serum starvation the cells were incubated for 20 hours with medium alone (sample 1) or in presence of LPS (100 ng/ml; samples 2-6) and subjected to flow cytometric analysis of cell surface phosphatidyl serine as described in “METHODS”. These data represent mean ± s.e. of three independent experiments. Asterisk* denotes a statistically significant difference (p<0.05) from positive control (M/LPS).