FIGURE 5. Similar CTL mediated killing responses in spleens of vaccinated BLT1^+/+ or BLT1^−/− animals.

The CTL in vivo killing assay was performed in groups of WT and BLT1^−/−mice as described in methods. The mice were vaccinated with 50 μg of E7_49-57 peptide + 10 μg of MPL-A in 200 μl of PBS. A group of naïve mice was also used as control. (A). Representative histograms of CFSE labeled targets. The killing levels of target cells in WT and BLT1^−/− mice immunized with the E7_49-57 peptide+ MPL-A vaccine as described in methods. Cumulative levels of killing activity (n=4) of peptide immunized (B). No functional defect was observed in killing activity of CTLs in BLT1^−/− mice.