Table 2.
Proteomic analysis of HEK lysate
| HCD energy | Proteins | Peptides | Spectra Identified | Unfiltered Spectra | % spectra identified |
|---|---|---|---|---|---|
| HCD20 (1) | 2672 | 18259 | 26879 | 183575 | 14.64% |
| HCD20(2) | 3271 | 31153 | 45331 | 198324 | 22.86% |
| HCD20(3) | 2830 | 24908 | 36416 | 182074 | 20.00% |
| 20 (sum) | 3743 | 39383 | 99849 | 563973 | 17.70% |
| HCD25 (1) | 3550 | 35952 | 51677 | 206399 | 25.04% |
| HCD25 (2) | 3357 | 33029 | 49636 | 204511 | 24.27% |
| HCD25 (3) | 2849 | 26699 | 41183 | 178234 | 23.11% |
| 25 (sum) | 4025 | 49095 | 130621 | 589144 | 22.17% |
| HCD30 (1) | 3548 | 38895 | 55713 | 203659 | 27.36% |
| HCD30 (2) | 3143 | 32685 | 48046 | 181652 | 26.45% |
| HCD30 (3) | 2535 | 22289 | 38796 | 159180 | 24.37% |
| 30 (sum) | 3939 | 49171 | 133589 | 544491 | 24.53% |
| Stepped (1) | 3587 | 37061 | 53342 | 203191 | 26.25% |
| Stepped (2) | 3341 | 35345 | 50381 | 186675 | 26.99% |
| Stepped (3) | 2293 | 17521 | 29961 | 167362 | 17.90% |
| 20, 25, 30 (sum) | 4032 | 49146 | 125321 | 557228 | 22.49% |