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. Author manuscript; available in PMC: 2013 Nov 3.
Published in final edited form as: Biomaterials. 2012 May 9;33(22):10.1016/j.biomaterials.2012.04.035. doi: 10.1016/j.biomaterials.2012.04.035

Fig. 6.

Fig. 6

(a) Protein homogenates (n = 5 per group, same as used in Fig. 5a) from whole visceral fat pads were analyzed for Ucp1 protein content by western blot and normalized by ponceu red staining. (b) Lack of a correlation between Ucp1 and GFP protein levels in visceral fat pads isolated from the control and GWT-treated mice. N.S., not significant, ANOVA. (c) Positive correlation between Ucp1 and GFP protein levels in visceral fat pads isolated from the control and GKO-treated mice. Significance levels were determined using one way ANOVA. (d) Inverse correlation between Ucp1 protein levels and lipid content (g lipid/g protein) in visceral fat pads isolated from the control and GKO- treated mice. Significance levels were determined using one way ANOVA. (e) Representative Ucp1 staining (brown, examples of Ucp1 staining are indicated by arrows) of paraffin embedded visceral fat from mock-injected, GWT- and GKO microcapsules injected mice at 10× magnification. Microcapsules indicated by ‘C’, the areas of host tissues as ‘H’. Selected Ucp1-positive areas of microcapsules and host adipocytes are shown in at 40× magnification. Numerous Ucp1-positive clusters of cells were seen only in GKO-capsule injected areas. The identity of encapsulated cells was confirmed by green fluorescence (data not shown).