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. Author manuscript; available in PMC: 2013 Nov 3.
Published in final edited form as: Brief Funct Genomics. 2010 Jul 2;9(4):281–293. doi: 10.1093/bfgp/elq017

Figure 4. Targeted meiotic recombination (TAMERE).

Figure 4

Recombination between loxP sites located in trans at non-allelic positions on homologous chromosomes can be induced by Cre in vivo. Triple transgenic males carrying the loxP site insertions on paternal (black) and maternal (gray) homologues of a given chromosome and the Sycp1-Cre transgene will undergo a Cre-mediated recombination during chromosome pairing at meiosis I (MI) at high frequencies. Following meiosis II (MII) mature gametes carrying either the deletion in the intervening sequence (Del) or its duplication (Dp) can be recovered and these new mutations can established in a mouse line by direct breeding.