Table 2.
Condition | Biofilm formation (n = 26)
|
||||||
---|---|---|---|---|---|---|---|
Weak No. Strains | OD + SD | Moderate No. Strains | OD + SD | Strong No. Strains | OD + SD | ||
Temperature (°C) | 12 | 19* | 0.39 ± 0.21 | 7 | 0.63 ± 0.09 | – | – |
15 | 14 | 0.51 ± 0.15 | 12 | 0.79 ± 0.12 | – | – | |
20 | – | – | 26 | 0.83 ± 0.25 | – | – | |
25 | – | – | 5 | 1.10 ± 0.19 | 21* | 1.69 ± 0.24 | |
28 | – | – | – | – | 26 | 1.97 ± 0.29 | |
30 | – | – | 26 | 0.78 ± 0.20 | – | – | |
32 | 26 | 0.19 ± 0.08 | – | – | – | – | |
a NaCl (%) | 1 | – | – | – | – | 26 | 1.42 ± 0.44 |
2 | – | – | – | – | 26 | 1.57 ± 0.48 | |
3 | – | – | – | – | 26 | 1.60 ± 0.53 | |
4 | – | – | – | – | 26 | 1.61 ± 0.60 | |
5 | – | – | 20* | 1.13 ± 0.09 | 6 | 1.03 ± 0.38 | |
6 | 4 | 0.42 ± 0.07 | 19* | 0.95 ± 0.40 | 3 | 1.23 ± 0.06 | |
7 | 26 | 0.41 ± 0.38 | – | – | – | – | |
8 | 26 | 0.03 ± 0.01 | – | – | – | – | |
9 | 26 | 0.01 ± 0.001 | – | – | – | – | |
Agitation (rpm) | 0 | – | – | – | – | 26 | 1.85 ± 0.39 |
60 | – | – | 9 | 1.17 ± 0.12 | 17* | 1.77 ± 0.46 | |
130 | 8 | 0.47 ± 0.29 | 10 | 1.20 ± 0.18 | 8 | 1.75 ± 0.32 | |
240 | 19 | 0.32 ± 0.11 | 7 | 0.73 ± 0.15 | – | – | |
MRM (Dilution factor) | Undiluted | 3 | 0.37 ± 0.08 | 21* | 0.84 ± 0.14 | 2 | 1.08 ± 0.07 |
1:2 | – | – | 17* | 1.08 ± 0.11 | 9 | 1.73 ± 0.10 | |
1:4 | 26 | 0.34 ± 0.28 | – | – | – | – | |
1:8 | 26 | 0.15 ± 0.06 | – | – | – | – | |
1:16 | 26 | 0.03 ± 0.01 | – | – | – | – |
1% = 10 parts per thousand
Significant difference within strains (p < 0.05) calculated using the Welsch step-up procedure.