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The I-Ag7/KS20 tetramer stains specifically the KS20-reactive T cell clone BDC-5.2.9. The T cell clones BDC-2.5, BDC-5.2.9 and BDC-10.1 were expanded in subcultures and stained with I-Ag7/CLIP, I-Ag7/KS20 or I-Ag7/2.5mi tetramers. After 2h, cells were stained with anti-CD4 APC. Gates are set on a CD4+/lymphocyte gate and histogram overlays represent tetramer staining levels on each T cell clone. Data is representative of three independent experiments.
