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. Author manuscript; available in PMC: 2013 Nov 3.
Published in final edited form as: FEBS J. 2013 Feb 24;280(6):10.1111/febs.12147. doi: 10.1111/febs.12147

Fig. 3.

Fig. 3

Directed chemical evolution of a selective affinity medium for Hsp90. SDS/PAGE silver stain showing the effects of different side chain modifications on Hsp90 recovery and recovery of nonspecifically bound proteins. Selectivity towards Hsp90 was demonstrated by inclusion of 1 mM of the free ligand (+) in the tissue extract prior to mixing with affinity resin. Mass spectrometry was used to define all of the bound protein. In the azo-6-PEG resin bound proteins were eluted with 25 mM sodium dithionite in NaCl/Pi. PEG, polyethylene glycol. Figure reproduced from [47].