Figure 1.

FluoZin-3 detects oxidant-mobilized Zn²⁺ in cultured primary cortical neurons. Panels A–C show representative traces from neurons loaded with FluoZin-3 and exposed to H₂O₂ (1 mM), MIT (100 μM), or DTDP (30 μM) for 10 min. A washout period (5 min) followed the oxidant treatment, then TPEN (5 μM) was added at the time indicated by the arrow. Panel D shows average fluorescence change (F/F₀ as mean ± S.E.) for the three oxidants, each at two concentrations. Also, similar experiments for solvents DMSO and ethanol (0.3% for each) are shown. Each experiment was performed on at least three different culture preparations. The total number of neurons, n, for each condition is as follows: H₂O₂ (100 μM), 134; H₂O₂ (1 mM), 195; DTDP (10 μM), 130; DTDP (30 μM), 115; MIT (30 μM), 90; MIT (100 μM), 78; DMSO, 76; EtOH, 69.