Figure 4.

Zn²⁺ mobilization requires elevated [Ca²⁺]_i and is partially dependent on ROS accumulation. Mean fura-2FF (solid) and FluoZin-3 (dashed) traces from neurons exposed to glutamate + glycine only (Panel A, reproduced from panel 2E for ease of comparison) or glutamate + glycine in Ca²⁺-free buffer (B), or with aristolochic acid (100 μM) (C), L-NAME (1 mM) (D), or FCCP (0.75 μM) (E). FCCP was applied for 5 min during the glutamate + glycine stimulus, while L-NAME and aristolochic acid were applied for 3 min before, during, and 3 min after glutamate + glycine. Experiments were concluded with a 5 min treatment with TPEN (5 μM), as indicated. Traces were averaged from 20–30 neurons from a single coverslip, and similar results were obtained from neurons from at least three different culture dates. See figure 5 for summary data.