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. Author manuscript; available in PMC: 2013 Nov 3.
Published in final edited form as: Recent Pat Biotechnol. 2013 Apr;7(1):71–81. doi: 10.2174/1872208311307010007

Fig. 8. EM-Stim promotes C2C12 proliferation and myogenic differentiation.

Fig. 8

A) Representative phase contrast images of C2C12 myoblasts cultured 72 h in DIFF that were exposed for 12 h (60–72 h) to control, ES, EM, and ES + EM conditions. B) Essentially the same data shown in panel A, but at 120h. Here cells were stimulated from 96 h to 108 h. C) Summarized cell area data for the myotubes at 120 h, after the 2 bouts of stimulation protocols. D) Total protein content of myotubes at 120h, after the 2 bouts of stimulation protocols. E) α-MHC qRT-PCR summary data for all 4 experimental conditions. In summary while both ES and EM had similar enhancing effects on myogenic differentiation, combination of ES + EM produced synergistic effects in enhancing myogenic differentiation. The black bars on each figure are a calibration bar representing 100μm.