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. 2010 Dec 23;4(1):98–105. doi: 10.1111/j.1751-7915.2010.00230.x

Figure 3.

Figure 3

Construction of B. subtilis SCK6. The sequence between two homologous arms (lacA′ and ′lacA) was replaced by the xylose‐inducible comK expression cassette from the linearized pAX01‐comK via a double‐crossing‐over recombination. X indicates one cross‐over event. lacA′ and ′lacA, upstream and downstream sequences of the B. subtilis lacA gene respectively; erm, erythromycin resistance gene; comK, B. subtilis comK gene; pxylA, xylose‐inducible promoter of xylA gene; xylR, B. subtilis xyl operon repressor; tlacA, terminator of B. subtilis lacA gene; t1t2 and t0, terminators of lambda phage.