Table 2.
Strains and plasmids.
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| E. coli | ||
| JM109 | recA1, supE44 endA1 hsdR17 (r−k, m+k) gyrA96 relA1 thi (lac‐proAB) F'[traD36 proAB+lacIqlacZ ΔM15] | Sambrook and Russel (2001) |
| BL21 Star (DE3) | F‐ompT hsdSB (rB‐mB‐) gal dcm rne131 (DE3) | Invitrogen, Carlsbad, CA |
| B. subtilis | ||
| 168 | trpC2 | Burkholder and Giles (1947) |
| 1A751 | his nprR2 nprE18 ΔaprA3 ΔeglS102 ΔbglT bglSRV | Wolf et al. (1995) |
| SCK6 | ErmR, 1A751 derivate, lacA::PxylA‐comK | This work |
| Plasmids | ||
| pP43NMK | AmpR, KmR, E. coli–B. subtilis shuttle vector | Zhang et al. (2005) |
| pP43N‐Bscel5 | AmpR, KmR, pP43NMK derivate with Bscel5 gene cloned | This work |
| pNW33N | CmR, E. coli–B. subtilis shuttle vector | Bacillus Genetic Stock Center |
| pNWP43N‐Bscel5 | CmR, pNW33N derivate, with Bscel5 expression cassette cloned | This work |
| pAX01 | AmpR, ErmR, B. subtilis integrative vector, xylose‐inducible promoter | Hartl et al. (2001) |
| pAX01‐comK | AmpR, ErmR, pAX01 derivate with comK cloned | This work |
| pET20b | AmpR, overexpression vector containing T7‐dependent promoter | Novagen, Madison, WI |
AmpR, ampicillin resistance; CmR, chloramphenicol resistance; ErmR, erythromycin resistance; KmR, kanamycin resistance.