Figure 3.

Schematic representation of the coupling between an in silico model of microbial metabolism (left) and a subsurface reactive transport model (right). The coupling can be in the form of either direct calls to the in silico model during execution of the reactive transport model or prior simulation of a large number of scenarios using the in silico model to generate a look‐up table that is then referenced during execution of the reactive transport model. In either case, flux constraints from the reactive transport model dictate the output from the in silico model, which is in turn fed back to the reactive transport model in terms of reaction fluxes (including biomass yields).