Fig. 1.

STORM imaging reveals distinct organization of actin filaments in the axons and dendrites of neurons. (A) Conventional fluorescence image of actin (green) and a dendritic marker, MAP2 (magenta), in a cultured hippocampal neuron fixed at 7 DIV. (B) 3D STORM image of actin in a dendritic region corresponding to the white box in (A). The z-positions in the STORM image are color-coded according to the color scale, with violet and red indicating positions closest to and farthest from the substratum, respectively. (C) Magnification of the region inside the red box in (B). The yz cross-section corresponding to the white-boxed region is shown in the inset. (D) Conventional fluorescence image of actin (green) and MAP2 (magenta) in a neuron fixed at 12 DIV. (E) 3D STORM image of actin in a region containing axons (devoid of the dendritic marker MAP2), corresponding to the yellow box in (D). The yz cross-sections corresponding to the white-boxed regions are shown in the insets. The 3D STORM image of a region containing a dendrite of this neuron is shown in Fig. S1B (28). (F) Conventional fluorescence image of actin (green) and an axon initial segment marker, NrCAM (magenta), in a neuron fixed at 9 DIV. (G) 3D STORM image of actin in a region containing the axon initial segments, corresponding to the dashed yellow box in (D).