Exploratory analysis of Vibrio scatter patterns grown on BHI‐NaCl plates: (A) Principal component plot, (B) Independent component plot, (C) Kernel principal component plot. (D) Linear discriminant plot showing separation of Vibrio classes in linear space. For the purpose of supervised classification four classes were created: Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, and ‘Mixture’ class containing colonies of Vibrio alginolyticus, Vibrio anguillarum, Vibrio mimicus and Vibrio orientalis. The classifiers were optimized to detect and recognize patterns of V. cholerae, V. parahaemolyticus and V. vulnificus.