Table 5.
Plasmids used in this study.
| Plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| pGEM‐T Easy | Apr, ColE1 origin | Promega |
| pNK1 | Apr, contains a 984 bp XhoI PCR fragment that located upstream of tolC. | This study |
| pNK2 | Apr, contains a 912 bp XhoI/BamHI PCR fragment located downstream of tolC | This study |
| pPS858 | Source of the Gm‐GFP cassette | Hoang et al. (1998) |
| pNK3 | Apr, Gmr, 1843 bp Gm‐GFP cassette from pPS858 cloned into BamHI‐linearized pNK2 | This study |
| pNK4 | Apr, Gmr, 2755 bp SpeI‐XhoI GFPGm‐downstream fragment from pNK3 cloned into SpeI‐XhoI‐linearized pNK1 | This study |
| pCAM‐MCS | Apr, pCAM140‐derivative without mini‐Tn5, contains the MCS of pBluescript II SK (+) | Burse et al. (2004) |
| pNK5 | Apr, Gmr, 3739 bp ScaI‐EcoRI upstream‐GFPGm‐downstream fragment cloned into EcoRI‐linearized pCAM‐MCS | This study |
| pNK6 | Apr, contains a 2630 bp PCR fragment carrying the tolC gene, upstream and downstream of the gene | This study |
| pNK7 | Cmr, contains a 2.6 kb fragment carrying tolC of E. amylovora cloned in pBBR1MCS by SpeI and SacII in opposite direction of lacZ | This study |
| pNK8 | Cmr, contains a 4.9 kb fragment carrying acrAB of E. amylovora cloned in pBBR1MCS by SpeI and SacI in opposite direction of lacZ | This study |
Ap, ampicillin; Gm, gentamicin; Km, kanamycin; Cm, chloramphenicol; GFP, green fluorescent protein.