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Oligonucleotide primers used in this study.
| Primer | Nucleotide sequence (5′‐3′)a |
|---|---|
| Ea‐tolC‐FwdI | GCTCACCACATGCACAAG |
| Ea‐tolC‐RevI | CTCGAGAGTGTTGCTGTTAGAGCCAC |
| Ea‐tolC‐FwdII | GCCAAATTCAGCCACGCA |
| Ea‐tolC‐RevII | CTCGAGGATCCGCAAGTGAACAGCTCGAAG |
| Ea‐acr‐Com‐Fwd | CGAGAGCTCCGCCAGTGACGTATTAGC |
| Ea‐acr‐Com‐Rev | GATACTAGTCGGTATAGTAAACGTGCG |
Restriction sites incorporated into primers are underlined: CTCGAG, XhoI; GGATCC, BamHI; GAGCTC, SacI; ACTAGT, SpeI.
