Table 2.
Characterization of γ-PGA produced by the four E. coli recombinant strains
| Strains | Yield (g l−1) | D-glutamate content (%) | Molecular weight (× 104Da) |
|---|---|---|---|
| E. coli-pTrcLpgs (109LP)a | 0.376 ± 0.021 | 2.46 ± 0.32 | 3.23 ± 0.26 |
| E. coli-pTrcNpgs (109NP)a | 0.308 ± 0.025 | 3.28 ± 0.27 | 3.74 ± 0.38 |
| E. coli-pTrcLRP (109LRP)a | 0.517 ± 0.027 | 8.96 ± 0.53 | 5.09 ± 0.33 |
| E. coli-pTrcNRP (109NRP)a | 0.349 ± 0.016 | 9.33 ± 0.47 | 5.83 ± 0.42 |
| E. coli-pTrcLpgs (109LP)b | 0.558 ± 0.018 | 3.01 ± 0.45 | 5.35 ± 0.28 |
| E. coli-pTrcNpgs (109NP)b | 0.533 ± 0.022 | 5.94 ± 0.66 | 5.89 ± 0.55 |
| E. coli-pTrcLRP (109LRP)b | 0.645 ± 0.016 | 18.13 ± 1.36 | 6.23 ± 0.46 |
| E. coli-pTrcNRP (109NRP)b | 0.603 ± 0.033 | 19.59 ± 0.98 | 6.95 ± 0.79 |
Carbon source of glucose.
Substrate of L-glutamate.