Table 2.

Effect of Fe³⁺ on flux distribution of NADPH and NADH

	Flux distribution of NADPH generation			Generation and consumption of NADH
	HMP/TCA (%)a	HMP/(HMP + TCA) (%)b	Total NADPH fluxc	From EMP (%)d	From TCA cycle (%)e	Forming glycerol (%)f	Total NADH fluxg
Control	28.6	22.3	3.12	52.1	45.3	5.48	6.32
Fe3+	17.8	15.1	3.95	73.2	24.2	4.13	7.83
Variation	-10.8		+26.6%	+40.5			+23.9%

^aHMP/TCA = (r₁₇/3)/(r₁₁/6) × 100%, flux ratio of NADPH generated through HMP (r₁₇) and isocitrate dehydrogenation in TCA cycle (r₁₁).

^bHMP/(HMP + TCA) = (r₁₇/3)/(r₁₁/6 + r₁₇/3) × 100%, proportion of NADPH generated through HMP (r₁₇) in the total flux of NADPH (through the PPP and the TCA cycle).

^cNADPH flux generated through reactions r₁₇ and r₁₁.

^dProportion of NADH flux generated through EMP (r₅) in the total NADH flux.

^rProportion of NADH flux generated by the TCA cycle (r₈ + r₁₂ + r₁₃) in the total NADH flux.

^fProportion of NADH flux to form glycerol through PDC pathway (r₄) in the total NADH flux.

^gStandardized by glucose consumption.