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. 2009 Dec 20;3(1):48–58. doi: 10.1111/j.1751-7915.2009.00135.x

Figure 1.

Figure 1

Activity screens for substrate‐specific variants of 3A6 saturation mutagenesis libraries. Identical copies of 96‐well microtiter plates containing crude cell lysates of saturation mutagenesis libraries Lys281X, Asp282X, Y315X, Asn316X and Lys317X were screened for activity with pNPC2 and MF. Activities for both substrates are plotted as rate of change of absorbance at 405 nm (for pNPC2) and 550 nm (for MF) per minute and corrected for bacterial cell growth. Subpopulations with altered substrate preference are explicitly shown: B, D and E correspond to CE‐selective mutants, whereas A, C and F correspond to FAE‐selective mutants.