Table 2.
Results of kinetic resolutions of substrates 1b–3b in preparative scale (in the presence of 10% DMSO).
| Substrate | Esterase | kUa | T (°C) | t (h) | eeSc (%) | eePd (%) | Ce (%) | Ee | Configuration |
|---|---|---|---|---|---|---|---|---|---|
| 1b | CL1 | 32b | 20 | 3 | 98 | 87 | 47 | > 200 | (−), R |
| 1b | A3 | 4b | 20 | 3 | 72 | 59 | 45 | 11 | (+), S |
| 2b | 8 | 6b | 20 | 1.5 | 92 | 56 | 62 | 11 | (−), R |
| 2b | CE | 3b | 30 | 8 | 82 | 97 | 46 | > 100 | (+), S |
| 3b | 8 | 3b | 30 | 8 | 97 | 95 | 50 | > 100 | (−), R |
| 3b | CE | 1b | 30 | 8 | 69 | > 99 | 41 | > 200 | (+), S |
a.
Determined using p‐nitrophenyl acetate.
b.
Substrate (40 mM).
c.
Determined by GC analysis.
d.
Determined by HPLC analysis.
e.
Calculated according to Chen and colleagues (1982).